RNA Recognition and Regulation of the AU - rich RNA Binding Proteins : HuR , TTP and BRF
نویسندگان
چکیده
RNA Recognition and Regulation of the AU-rich RNA Binding Proteins: HuR, TTP and BRF1 by Matthew Burk Friedersdorf Department of Pharmacology and Molecular Cancer Biology Duke University Date:_______________________ Approved: ___________________________ Jack Keene, Supervisor ___________________________ Matthais Gromeier ___________________________ Jeffrey Rathmell ___________________________ Dennis Thiele ___________________________ David Macalpine An abstract of a dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Pharmacology and Cancer Biology in the Graduate School of Duke University 2011 Copyright by Matthew Burk Friedersdorf 2011
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TIAR and HuR are mRNA-binding proteins that play important roles in the regulation of translation. They both possess three RNA recognition motifs (RRMs) and bind to AU-rich elements (AREs), with seemingly overlapping specificity. Here we show using SPR that TIAR and HuR bind to both U-rich and AU-rich RNA in the nanomolar range, with higher overall affinity for U-rich RNA. However, the higher a...
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